Contact no : 03038366232 (PAKISTAN)
Email: Khanamir6@hotmail.com

 

TEACHING EXPERIENCE AND RECENT POSTS

Sept 2010  to date    
Assistant professor at Quaid-i-Azam University Islamabad

Working as an assistant professor and teaching M.Sc andM.Phil courses in the department of biochemistry. Also, supervise students for their M.Phil projects.

 

March 2010   Visiting Scholar at Quaid-i-Azam University Islamabad

 

June 2010
Invited by department of biochemistry to conduct seminar, lectures etc. in the fields of stem cells and proteomics. Trained PhD and M-Phil students in the 2-D PAGE and tissue culture techniques

 

POSTDOCTORAL RESEARCH ASSOCIATE

2008-2009       Research associate in Stem cell Biology School of Medicine University of Leeds
Grown H1 and Hues1 human embryonic stem cell lines for Professor Helen Picton’s Reproduction and Early DevelopmentResearch group to measure the expression and methylation of imprinted genes in Hues1 and H1 at different passages using an imprinted gene microarray and pyrosequencing.

 

EDUCATION AND QUALIFICATIONS

    • PhD “ Proteomics analysis of human embryonic and mesenchymal stem cells”

University of Leeds (UK)
Supervised by Professor John B.C. Findlay.

  • Passed PhD degree on 25th November 2008. During the research project, grew and set up undifferentiated human embryonic stem cell lines (H1 and Hues1). Gained extensive experience in the undifferentiated growth and passage of the hES cell lines on feeder layers. Also gained experience in the differentiation of H1 and Hues1 cell lines into embryoid bodies.
  • The undifferentiated growth of the cell lines was frequently validated with specific pluripotent markers such as OCT4 and alkaline phosphatase. The expression of OCT4 was confirmed by western blotting.
  • Also grown and differentiated pure mesenchymal stem into adipocytes.
  • Extracted proteins from total and membrane enriched fractions of human embryonic stem cell line (Hues1) and mesenchymal stem cells, separated them by 2D-PAGE and sequentially stained them with Pro-Q Diamond and Sypro Ruby. Proteins that were uniquely expressed and common but differentially regulated were identified by MALDI-TOF. A few of these protein spots were also identified by LC/MS/MS. Consequently, have gained in-depth practical experience of proteins separation by 2-D PAGE, analysis of the gels images by PD-Quest software and tryptic digestion of protein spots and their identification by MALDI-TOF.
  •  Phosphorylated proteins that were uniquely expressed, most of the common and common but differentially regulated between the two stem cells types were also identified by MALDI-TOF. A few of these identified proteins are involved in cell signalling pathways and consequently a few pathways that are involved in the self-renewal of  mesenchymal and embryonic stem cells have been elucidated.
  •  Collaborated with Professor Alan Handyside who is the director of Bridge fertility clinic in London.

 

2001-2004       BSc (honour) 2:1, Human Genetics
University of Leeds (UK)
Modules studied: Human Genetic disorders, Understanding Human Inherited Diseases, Cancer Genetics, Genetics of Immunology, Animal Developmental Biology 3, Human Genome Project. Bioinformatics, Cell signalling, Eukaryotic Genetics and cloning, Prokaryotic Genetics, Animal Developmental Biology 2, Human Population, Human Genetics, Enhanced skills for Biologist, Introduction to Immunology, Genome Structure, Biochemistry-Molecules, Cells and Organism, Eukaryotic Metabolism, Human Physiology and Human Anatomy.  Undergraduate research project involved PCR, cloning and transformation.

1996-1998       A-Levels: Biology (B), Physics (B) and Mathematics (E)
Parklane College Leeds.

1993-1996       G.C.S.E’s: Chemistry (B), Mathenatics (B)and English (B)

A-level Chemistry (B)
BradfordCollege

 

            TEACHING AND ADMISTRATIVE EXPERIENCE

2004-2008       Post graduate demonstrator, University of Leeds

Demonstrated various Undergraduate Biochemistry and Genetics modules.Responsible for more than 25 students and for explaination of the practicals and any COSH issues.Furthermore during the practical, helped module managers by marking the practicals scripts.
.

 

 

PUBLICATIONS

1): Amir A. Khan, Jeffrey N. Keen, and John B. C. Findlay: Proteomics and phospho proteomics analysis of Human Embryonic and Mesenchymal Stem Cells. Journal of Proteome Research.Submitted.

2): Huntriss, J., Hinkins, M., Khan, A.A., Picton, H.M.Microarray Analysis of Imprinted Gene Expression in Human Embryonic
Stem Cells: Imprinted Genes as Biomarkers for Assessing Epigenetic
poster presentation atEuroEpiStem: European Epigenomics& Stem Cells-2009 Meeting.

3): Huntriss J, Woodfine K,  Huddleston J, Murrell A,  Rutherford A, Elder K, Khan A.A, Hemmings K,  Picton H.M: Quantitative Analysis of DNA Methylation of Multiple Imprinted Genes in Single Human Blastocysts by Pyrosequencing.Fertility and sterility. Volume 95, Issue 8, 30 June 2011, Pages 2564-2567.

4) and (5): Khan M, Khan R and Khan AA: Polymorphism in the promoters of oct4 and Sox2 genes in thePakhtoon population of Pakistan: Manuscripts in preparation

 

COURSES ATTENDED

Human Embryonic Stem Cell culture in July 2005. The course was arranged by University of Sheffield.

CONFERENCES ATTENDED

 Presentation of a poster in Mesenchymal stem cells conference in June 2007, held at University of York.

IT SKILLS

Extensive knowledge of Microsoft Office programmes and proficient in the use of Word, PowerPoint and Excel.Also experienced with PD-Quest Software for the analysis of 2-D gels.

WORK AND OTHER EXPERIENCE

    • Interpreter (part-time)

Home office UK (Leeds and Liverpool)
Employed by Home Office as an interpreter for Pashto and Urdu languages.Used to interpret to Afghan asylum seekers from English to Pashto or vice versa in the interviews.

 

 

 

Contact Information

Biochemistry Department QAU Islamabad Pakistan
Phone: 05190643169
E-mail: baqi.qau@gmail.com

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